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  • M9808 - Anti-Muscarinic Acetylcholine Receptor (M1) antibody produced in rabbit

M9808 Sigma

Anti-Muscarinic Acetylcholine Receptor (M1) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym: Acetylcholine Receptor antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Neurotransmission, Antibodies to Neurotransmitter Receptors, MUE-MZ,
species reactivity   rat, mouse
application(s)   immunohistochemistry: suitable
  western blot: 1:200 using rat brain lysates
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... CHRM1(1128)
rat ... Chrm1(25229)
biological source   rabbit
conjugate   unconjugated



GST fusion protein of a part of the i3 intracellular loop of human M1 muscarinic acetylcholine receptor (mAChR) corresponding to amino acid residues 227-353.1, 2

General description

Muscarinic cholinergic receptor 1 (M1) is seen in the forebrain of rats.

Physical form

Lyophilized from a solution of phosphate-buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide.


Anti-Muscarinic acetylcholine receptor (M1) antibody produced in rabbit has been used in immunohistochemistry and Western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Biochem/physiol Actions

Studies have shown that activation of muscarinic cholinergic receptor 1 (M1) in rats leads to changes in the endotoxin tolerance in the liver.

Price and Availability

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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