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M9901 Sigma-Aldrich

Mutanolysin from Streptomyces globisporus ATCC 21553

lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified



General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.


Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.


1000, 5000, 10000, 50000 units in glass bottle

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Unit Definition

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

Fiberfrax is a registered trademark of Unifrax Corp.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles


Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products. The Continuous Spectrophotometric Rate Determination (A600, Light path = 1 cm) is based on the following reaction:

GenElute Bacterial Genomic DNA Kit Protocol

Our GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to isolate pure DNA from a variety of cultured bacteria. The kit contains all of the reagents needed to isolate and purify...
Keywords: AGE, Amplification, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Pulsed field gel electrophoresis

GenElute Bacterial Genomic DNA Kit, Experienced User Protocol

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information.

Related Content

Enzyme Explorer Key Resources: Lysing Enzymes

Cell Lysis Enzymes – How to tear down the Wall Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your...
Keywords: Amplification, Biochemistry, Cell culture, Cell disruption, Culture media, Enzymology, Metabolites, Molecular biology, PAGE, Purification, Sample preparations, Sequencing

Peer-Reviewed Papers


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M3440 Mutanolysin Assay Substrate, (Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.)

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