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MAC4L Sigma-Aldrich

MetaPolyzyme

lyophilized powder

Synonym: Multilytic Enzyme Mix

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Description

General description

Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.

Application

MetaPolyzyme is a mixture of 6 enzymes that is intended for isolation of total DNA for metagenomics studies. Adapted from an initial formulation devised by S. Tighe,2 MetaPolyzyme was evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG). Test data on the use of MetaPolyzyme is accessible at the 2017 ABRF MGRG Poster.

Biochem/physiol Actions

Microbes can be difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted by using lysing enzymes (such as lyticase or chitinase) to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. MetaPolyzyme is intended for use in metagenomic studies, such as study of microbes and microbiomes of extreme and unique environments. MetaPolyzyme is intended for digestion of microbes for evaluation by whole genome shotgun sequencing for metagenomics and metatranscriptomic approaches.

Components

The enzymes in MetaPolyzme are:
• Achromopeptidase
• Chitinase
• Lyticase
• Lysostaphin
• Lysozyme
• Mutanolysin

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

Previously, the study of human microbiology was based on identification, isolation and culture of single microbes, such as bacteria, fungi and viruses. Scientists collected these organisms from patie...
Keywords: Amplification, Antibiotics, Antifungals, Cell culture, Cell disruption, Clinical, Diabetes, Digestions, Diseases, Environmental, Gas chromatography, Gas chromatography mass spectrometry, Gastrointestinal, Genetics, Genomics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Metabolomics, Microbiology, Nutrition, Obesity, Polymerization reactions, Proteomics, Respiratory, Sample preparations, Separation, Sequencing

Peer-Reviewed Papers
15

References

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