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MAK003 Sigma-Aldrich

Branched Chain Amino Acid Kit

sufficient for 100 colorimetric tests

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Description

General description

The branched-chain amino acids (BCAA), leucine, isoleucine, and valine, are the most common essential amino acids in proteins. The BCAA may function in both energy production and as nutrient signals. Dietary supplementation with BCAA may aid in the treatment of various disorders such as Amyotrophic Lateral Sclerosis (ALS), latent and chronic hepatic encephalopathies, and to prevent muscle atrophy in cancer patients. BCAA may increase the rate of protein synthesis and sensitize cells to insulin and insulin-like growth factor.

Application

Branched Chain Amino Acid Kit has been used to determine the concentration of hepatic branched-chain amino acids, in a study assessing the role of CREB regulated transcription coactivator 2 (CRTC2) in hepatic lipid homeostasis and obesity in mice.

Suitability

Suitable for measuring Branched Chain Amino Acids (BCAA’s) in a variety of biological samples.

Principle

In this assay, BCAA concentration is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the BCAA present. The BCAA Assay Kit provides a simple convenient means of measuring the BCAAs in a variety of biological samples.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Protocols

Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)

This assay protocol is suitable for the colorimetric detection of branched-chain amino acids, leucine, isoleucine, and valine, in cell and tissue culture supernatants, urine, plasma, serum, and other...
Keywords: Enzyme activity, Sample preparations

Peer-Reviewed Papers
15

References

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