MAK004 Sigma-Aldrich

Glutamate Assay Kit

sufficient for 100 colorimetric tests

  •  NACRES NA.84



General description

Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter. It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease. Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.


Glutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model. It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.


Suitable to determine glutamate concentration in a variety of samples.


Glutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.

Safety & Documentation

Safety Information

GHS09  GHS09
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Glutamine Metabolism is Dysregulated in Many Cancer Cells

Proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metab...
BioFiles v7 n4
Keywords: Aerobic, Cancer, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Metabolites, Phosphorylations, Transcription


Enzymatic Method for Determining Glutamate (Glutamate Assay)

This assay protocol is suitable for the colorimetric detection of glutamate in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Glutamate Assay Kit (...
Keywords: Sample preparations

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?