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MAK006 Sigma-Aldrich

Urea Assay Kit

sufficient for 100 colorimetric tests

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Description

General description

Urea is the major end product of nitrogen metabolism in most animals and is produced in a series of reactions in the liver called the urea cycle. In the urea cycle, ammonia is converted to urea, which is carried by blood to the kidneys for elimination from the body. High levels of urea in the blood may indicate renal failure. Urea levels may also be elevated in response to treatment with certain drugs such as corticosteroids or in response to decreased kidney filtration due to dehydration or congestive heart failure. Decreased blood urea levels can occur in response to liver disease or malnutrition.

Application

Urea assay kit has been used to measure the concentration of urea.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of urea concentration in a variety of samples such as serum, plasma, and urine, etc.

Principle

In this assay, Urea concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm) product, proportional to the Urea present.

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Protocols

Enzymatic Method for Determining Hydroxyproline

This assay protocol is suitable for the colorimetric detection of hydroxyproline in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Hydroxyproline A...
Keywords: Oxidations, Sample preparations

Enzymatic Method for Determining Urea

This assay protocol is suitable for the colorimetric detection of urea in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Urea Assay Kit (MAK006). U...
Keywords: Enzyme activity, Sample preparations

Peer-Reviewed Papers
15

References

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