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MAK018 Sigma-Aldrich

Maltose and Glucose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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Description

General description

Maltose is a disaccharide containing two glucose molecules with an a(1→4) glycosidic linkage. The enzyme α-D-glucosidase hydrolyzes maltose to its constituent monosaccharides. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Maltose can be derived from starch in food through the action of amylase. Maltose can be found in many food products, including beer, cereals, and pasta.

Application

Maltose and Glucose Assay Kit has been used to estimate maltose present in coral, coral larvae, Symbiodinium and seawater.

Suitability

Suitable for the direct detection of maltose and glucose in a variety of samples, including serum, plasma, food, or growth media.

Principle

The Maltose and Glucose Assay kit provides a simple and direct procedure for measuring maltose and glucose in a variety of samples, including serum, plasma, food, or growth media. In this kit, maltose is converted to two glucose units via α-D-Glucosidase. Glucose is further oxidized, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the maltose present. This kit has a linear detection range of 0.1-0.5 nmole maltose for the fluorometric assay and 1-5 nmoles maltose for the colorimetric assay.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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