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MAK020 Sigma-Aldrich

Fructose-6-Phosphate Assay Kit

sufficient for 100 fluorometric tests

  •  NACRES NA.84

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Description

General description

Fructose-6-phosphate (F6P) is a glycolytic pathway intermediate produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. F6P is further phosphorylated to fructose 1,6-biphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. F6P can also be shunted from glycolysis to the non-oxidative branch of the pentose phosphate pathway as a means of generative pentose phosphates for nucleotide synthesis. F6P levels are elevated in rapidly proliferating cells such as cancer cells. The Fructose-6-phosphate Assay Kit is a highly sensitive and simple fluorescence-based method of quantifying F6P in a variety of samples.

Application

Fructose-6-Phosphate Assay Kit has been used to measure fructose-6-phosphate (F-6-P) and glucose-6-phosphate (G6P).

Suitability

Suitable for quantifying Fructose-6-Phosphate in a variety of samples

Principle

Fructose-6-phosphate concentration is determined by a coupled enzyme reaction, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the fructose-6-phosphate present. Typical sensitivities for this kit are 0.01 to 0.5 nmoles of F6P.

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Articles

Aerobic Glycolysis and the Warburg Effect

The Warburg effect is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer ce...
BioFiles v7 n4
Keywords: Aerobic, Cancer, Cell proliferation, Clinical, Glycolysis, Phosphorylations, Transcription

Peer-Reviewed Papers
15

References

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