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MAK026 Sigma-Aldrich

Magnesium Assay Kit

sufficient for 100 colorimetric tests

  •  NACRES NA.84

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Description

General description

Magnesium, one of the most abundant trace metals in living organisms, contributes to a variety of biological functions including ATP and nucleic acid processing, energy metabolism, and enzymatic function. Magnesium forms complexes with multiple molecules such as phospholipids and ATP. Low serum levels of magnesium have been associated with metabolic syndrome, diabetes mellitus type 2, and hypertension, as well as, increased smooth muscle and platelet reactivity.

Application

Magnesium Assay Kit has been used to quantify magnesium levels in conditioned media.

Suitability

Suitable for quantitating magnesium concentrations in a variety of biological samples.

Principle

The Magnesium Assay kit provides a simple and direct procedure for measuring magnesium in a variety of samples. The magnesium concentration is determined by a coupled enzyme assay that takes advantage of the specific requirement of glycerol kinase for Mg2+, resulting in a colorimetric (450 nm) product proportional to the magnesium present. This assay gives a linear range of 3-15 nmoles of magnesium and exhibits no detectable interference with Fe2+, Cu2+, Ni2+, Zn2+, Co2+, Ca2+, and Mn2+.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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