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MAK043 Sigma

Cholesterol Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym: Cholesterol Assay Kit



Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Cholesterol is an important component of mammalian cell membranes where it functions in intracellular transport, cell signaling, and maintaining membrane fluidity. Within the blood, cholesterol circulates as both the free acid and as cholesterol esters. Controlling serum cholesterol has an important therapeutic role as elevated cholesterol levels are associated with the development of atherosclerosis and cardiovascular pathologies. Recent evidence suggests a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.

The Cholesterol Quantitation Kit can also be used to determine the concentration of free cholesterol, cholesteryl esters, or both (total) present in a sample. In this kit, total cholesterol concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the cholesterol present.


Suitable for use with cell and tissue culture samples, urine, plasma, serum, and other biological samples.

Price and Availability

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Safety & Documentation

Safety Information

NA 1993 / PGIII
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Can these kits be used with serum samples from mouse?
Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.
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Protocols & Articles
Peer-Reviewed Papers


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