• USA Home
  • MAK053 - Alcohol Dehydrogenase Activity Assay Kit

EMAIL THIS PAGE TO A FRIEND
MAK053 Sigma-Aldrich

Alcohol Dehydrogenase Activity Assay Kit

sufficient for 100 colorimetric tests

  •  NACRES NA.84

Purchase

Properties

Related Categories Assay Kits by Specialty Area, Blood and Urine Chemistry Assay Kits, Cell Biology, Enzymatic Activity Assay Kits, Enzymatic Kits for the Quantitation of Nutrients and Metabolites,
usage   sufficient for 100 colorimetric tests
storage temp.   −20°C
Gene Information   human ... ADH1A(124), ADH1B(125), ADH1C(126), ADH4(127), ADH5(128), ADH6(130), ADH7(131)
mouse ... ADH1C(11522), ADH4(26876), ADH5(11532), ADH6A(69117), ADH7(11529)
rat ... ADH1C(24172), ADH4(29646), ADH5(100145871), ADH6(310903), ADH6A(295498), ADH7(171178)

Description

General description

Alcohol dehydrogenases (ADH) are a family of enzymes that catalyzes the conversion of alcohols to aldehydes, with the concomitant reduction of NAD+ to NADH. In humans, there are nine isozymes of ADH, with the majority of ADH activity occurring in the liver. ADH family members are the primary enzymes involved in alcohol detoxification. Genetic variations in ADH enzymes result in differences in ADH activity and tolerances for alcohol, and may regulate susceptibility to alcoholism.

Application

Alcohol Dehydrogenase Activity Assay Kit has been used to determine the activity of alcohol dehydrogenase.

Suitability

Suitable for the detection of alcohol dehydrogenase in a variety of samples including tissue, cells and serum.

Principle

The Alcohol Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring ADH activity in a variety of samples. ADH activity is determined using ethanol as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of ADH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at 37 °C.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3316 9
Flash Point(F) 
53.6 °F - closed cup
Flash Point(C) 
12.0 °C - closed cup
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?