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MAK060 Sigma-Aldrich

Fumarate Assay Kit

sufficient for 100 colorimetric tests

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Description

General description

Fumarate is a tricarboxylic acid (TCA) cycle intermediate formed by the oxidation of succinate by succinate dehydrogenase. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. Deficiencies in fumarate hydratase are associated with increased cellular concentrations of fumarate and increased incidences of renal cell cancer. This is most likely due to fumarate-mediated upregulation of HIF1. The Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples.

Application

Fumarate Assay Kit has been used to determine the concentration of fumarate in samples.

Suitability

Suitable for the detection of fumarate in a variety of samples including tissue, cells and serum

Principle

Fumarate is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the fumarate present.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metab...
BioFiles v7 n4
Keywords: Aerobic, Cancer, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Metabolites, Phosphorylations, Transcription

Peer-Reviewed Papers
15

References

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