• USA Home
  • MAK061 - Isocitrate Assay Kit

EMAIL THIS PAGE TO A FRIEND
MAK061 Sigma-Aldrich

Isocitrate Assay Kit

sufficient for 100 colorimetric tests

  •  NACRES NA.84

Purchase

Description

General description

Isocitrate is a TCA cycle intermediate that is formed from citrate by the activity of the enzyme aconitase. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. In plants and lower organisms, isocitrate is the branch point from which the glyoxylate shunt operates. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. The Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples.

Suitability

Suitable for quantifying isocitrate in a variety of samples including tissue, cells, food and beverages

Principle

Isocitrate concentration is determined by a coupled enzyme assay, which results in a colorometric (450 nm) product, proportional to the isocitrate present. Typical sensitivities of detection for this kit are 1-20 nmoles (0.2 - 5 μg) of isocitrate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

Chiral 2-hydroxyglutarates (2-OHG) are important molecular signatures of their specific biochemical pathways and inborn errors of metabolism, for both healthy and diseased biological cells. For examp...
Roland Wöhlgemuth, Rudolf Köhling, Hugh Cramer
Reporter Volume 31.3
Keywords: Asymmetric synthesis, Cancer, Citric Acid Cycle, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Metabolites, Phase transitions, Reductions, Separation

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metab...
BioFiles v7 n4
Keywords: Aerobic, Cancer, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Metabolites, Phosphorylations, Transcription

LCMS Separation and Identification of 2-Hydroxyglutarates

Sigma-Aldrich has recently developed an application for the separation and identification of S-2-hydroxyglutaric acids and R-2-hydroxyglutaric acids by chiral LC-MS, using a CHIROBIOTIC® R Column (co...
Keywords: AGE, Cancer, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Reductions, Separation, Tools

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?