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MAK062 Sigma-Aldrich

IDH Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym: Isocitrate Dehydrogenase Activity Assay

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Description

General description

Isocitrate dehydrogenase (IDH) catalyzes the conversion of isocitrate to α-ketoglutarate. In eukaryotes, there are three isozymes of IDH, the mitochondrial IDH2 and IDH3, and the cytoplasmic/ peroxisomal IDH1. All three IDH family members require the presence of a divalent cation (Mg2+ or Mn2+) and either the electron-accepting cofactor NADP+ (IDH1 and IDH2) or NAD+ (IDH3) for their enzymatic activity. IDH1 and IDH2 mutations resulting in neomorphic enzymatic activity are found in certain cancers such as glioblastoma, acute myeloid leukemia, and colon cancer. This neoactivity shows a change in the substrate specificity resulting in the conversion of α-ketoglutarate to 2-hydroxyglutarate. Mutations in IDH family members are also associated with Ollier disease and Maffucci syndrome.

Application

IDH Activity Assay Kit has been used to determine the activity of isocitrate dehydrogenase in samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of isocitrate dehydrogenase (IDH) (NAD+ - dependent, NADP+ -dependent or both IDHs) activity in biological samples including tissue, cells and serum

Principle

The Isocitrate Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring NADP+-dependent, NAD+-dependent, or both NADP+ and NAD+-dependent IDH activity in a variety of samples. IDH activity is determined using isocitrate as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of IDH is the amount of enzyme that will generate 1.0 μmole of NADH or NADP per minute at pH 8.0 at 37 °C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

Chiral 2-hydroxyglutarates (2-OHG) are important molecular signatures of their specific biochemical pathways and inborn errors of metabolism, for both healthy and diseased biological cells. For examp...
Roland Wöhlgemuth, Rudolf Köhling, Hugh Cramer
Reporter Volume 31.3
Keywords: Asymmetric synthesis, Cancer, Citric Acid Cycle, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Metabolites, Phase transitions, Reductions, Separation

LCMS Separation and Identification of 2-Hydroxyglutarates

Sigma-Aldrich has recently developed an application for the separation and identification of S-2-hydroxyglutaric acids and R-2-hydroxyglutaric acids by chiral LC-MS, using a CHIROBIOTIC® R Column (co...
Keywords: AGE, Cancer, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Reductions, Separation, Tools

Peer-Reviewed Papers
15

References

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