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MAK063 Sigma-Aldrich

L-Carnitine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

  •  NACRES NA.84

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Description

General description

Carnitine is a metabolite that is synthesized from lysine and methionine. Carnitine is essential for fatty acid β-oxidation due to its role in fatty acid transport into the mitochondrial matrix via the carnitine/acylcarnitine shuttle. Carnitine exists in two stereoisomers but only the L-carnitine isomer is biologically active. Tissue levels of L-carnitine decrease with aging and decreased L-carnitine may contribute to age-related mitochondrial decline. The L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum.

Application

L-Carnitine Assay Kit has been used to determine the concentration of carnitine in serum samples.

Suitability

Suitable for the measurement of free L-Carnitine in biological samples such as tissue, cells and serum

Principle

L-carnitine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the L-carnitine present. Typical sensitivities of detection for this kit are 10 μM (colorimetric) and 1 μM (fluorometric). The normal range for serum L-Carnitine is between 10-70 μM.

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can these kits be usedwith serum samples from mouse?
Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount oftime they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles

Articles

Fatty Acid Synthesis and Metabolism in Cancer Cells

Proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sour...
BioFiles v7 n4
Keywords: Apoptosis, Cancer, Carboxylations, Catalysis, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Oxidations, Phosphorylations

Peer-Reviewed Papers
15

References

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