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MAK066 Sigma-Aldrich

Lactate Dehydrogenase Activity Assay Kit

sufficient for 500 colorimetric tests

Synonym: LDH Activity Assay Kit, LDH Assay, Lactic Dehydrogenase Activity Assay Kit

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Description

General description

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. LDH is also elevated in certain pathological conditions such as cancer. Quantification of LDH has a broad range of applications.

Application

Lactate Dehydrogenase Activity Assay Kit has been used for measuring the activity of lactate dehydrogenase (LDH) in the cell culture medium.
Lactate Dehydrogenase Activity Assay Kit has been used to determine the concentration of lactate dehydrogenase in samples.

Principle

In this kit, LDH reduces NAD to NADH, which is specifically detected by colorimetric (450 nm) assay. The LDH Activity Assay kit quantifies LDH activity in variety of biological samples. The assay is quick, convenient, and sensitive.

Suitability

Suitable for the detection of L(+)-Lactate in biological samples such as serum, plasma, cells, culture and fermentation media.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

D-2-Hydroxyglutarate (D2HG) Assay Kit
Protocols & Articles

Articles

Aerobic Glycolysis and the Warburg Effect

The Warburg effect is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer ce...
Keywords: Aerobic, Cancer, Cell proliferation, Clinical, Glycolysis, PAGE, Phosphorylations, Transcription

Peer-Reviewed Papers
15

References

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