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MAK090 Sigma-Aldrich

γ-Glutamyltransferase (GGT) Activity Fluorometric Assay Kit

sufficient for 100 fluorometric tests

Synonym: Gamma Glutamyltransferase Activity Fluorometric Assay Kit

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Properties

Related Categories Assay Kits by Specialty Area, Cardiology - Cardiovascular, Cell Biology, Enzymatic Activity Assay Kits, Enzymatic Kits for the Quantitation of Nutrients and Metabolites,
usage   sufficient for 100 fluorometric tests
storage temp.   −20°C
Gene Information   human ... GGT1(2678), GGT2(728441), GGT5(2687), GGT6(124975), GGT7(2686)
mouse ... GGT1(14598), GGT5(23887), GGT6(71522), GGT7(207182)
rat ... GGT1(116568), GGT5(296566), GGT6(408206), GGT7(156275)

Description

General description

γ-Glutamyltransferase (GGT, EC 2.3.2.2) is a membrane-bound protein that catalyzes the transfer of γ-glutamyl moieties to acceptor molecules such as amino acids or peptides. GGT plays a key role in the γ-glutamyl cycle, a critical pathway for glutathione homeostasis as well as the detoxification of xenobiotics. Serum GGT levels can be elevated in many pathophysiological conditions such as cardiovascular disease, chronic liver disease, and metabolic syndrome. Elevated serum levels of GGT can also be indicative of oxidative stress.

Application

γ-Glutamyltransferase (GGT) Activity Fluorometric Assay Kit has been used to analyze serum GGT activity.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of gamma-glutamyl transferase (GGT) in a variety of samples including tissue, cells and serum

Principle

The GGT Activity Fluorometric Assay kit provides a simple and direct procedure for measuring GGT activity in a variety of samples. GGT activity is determined by a coupled enzyme assay, in which the GGT transfers the γ-glutamyl group from quenched substrate L-γ-Glutamyl-7-amido-4-methylcoumarin (L--Glutamyl-AMC), liberating the fluorescent group, 7-Amino-4-Methyl Coumarin (AMC) (λex = 365 nm/λem = 460 nm) proportional to the GGT activity present. One unit of GGT is the amount of enzyme that will generate 1.0 μmole of AMC per minute at 37 °C.

Safety & Documentation

Safety Information

Flash Point(F) 
188.6 °F - closed cup
Flash Point(C) 
87 °C - closed cup
Protocols & Articles
Peer-Reviewed Papers
15

References

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