• USA Home
  • MAK107 - LCAT Activity Assay Kit

EMAIL THIS PAGE TO A FRIEND
MAK107 Sigma-Aldrich

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonym: Lecithin:cholesterol acyltransferase activity assay kit

Purchase

Description

General description

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Application

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.

LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for measuring phospholipase activity of LCAT in plasma or serum.

Principle

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λex=390nm/λem=470nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Kit component only

Description

Product #

Add to Cart

Substrate Reagent .1 mL    
READ Reagent 30 mL    
LCAT Assay Buffer 20 mL    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
49.5 °F
Flash Point(C) 
9.7 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Are products MAK107 and MAK108 compatible with mouse serum?
Yes, but please be aware of possible sample hemolysis. Depending on the method of mouse sample collection, hemolysis may be common, which can interfere with fluorescence emission via color quenching.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

Roar Assay Kits for Studies in Lipoprotein Metabolism

This technical article is a compilation of select fluorescence-based activity assays developed by Roar Biomedical now offered through Sigma-Aldrich in kit format. The methods are well-characterized, ...
Robert Brocia
Roar Biomedical, Inc.
Keywords: Addition reactions, Cardiovascular, Chromatography, Clinical, Detection methods, Drug discovery, Esterifications, Gas chromatography, High performance liquid chromatography, Separation, Thin layer chromatography

Protocols

LCAT Activity Assay Kit

The emission spectrum of the Substrate Reagent has two distinct peaks, 390 nm and 470 nm. The relative intensity of the peaks depends upon the concentration of hydrolyzed and intact substrate present...
Keywords: Titrations

Related Products

related product

Product #

Description

Add to Cart

MAK306 LCFC-LCAT Acyltransferase Activity Assay, Supplied by Roar Biomedical, Inc., sufficient for 100 fluorometric tests
57858 Sodium iodoacetate, BioUltra, ≥99.5% (NT)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?