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MAK109 Sigma-Aldrich

LPL Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonym: Lipoprotein lipase activity assay kit

  •  NACRES NA.84

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Description

General description

Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.

Application

LPL Activity Assay Kit may be used for detection of LPL activity in plasma samples of patients with hypertriglyceridemia.

Suitability

Suitable for the measurement of lipoprotein lipase activity in a variety of tissue samples

Principle

The LPL Activity Assay Kit includes a non-fluorescent substrate emulsion that becomes intensely fluorescent upon interaction with LPL and pre-hydrolyzed substrate for use as a standard to convert the fluorescence intensity reading to moles of reactant formed (λex=370nm/λem=450nm). The assay is not specific for LPL and will also detect hepatic lipase activity.

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Roar Assay Kits for Studies in Lipoprotein Metabolism

This technical article is a compilation of select fluorescence-based activity assays developed by Roar Biomedical now offered through Sigma-Aldrich in kit format. The methods are well-characterized, ...
Robert Brocia
Roar Biomedical, Inc.
Keywords: Addition reactions, Cardiovascular, Chromatography, Clinical, Detection methods, Drug discovery, Esterifications, Gas chromatography, High performance liquid chromatography, Separation, Thin layer chromatography

Protocols

LPL Activity Assay Protocol

The kit is sufficient for 100 assays in 200 µL total assay volume. Substrate Emulsion                                    0.125 mL Catalog Number MAK109A LPL Standard, 75.7 mM                         ...
Keywords: Sample preparations

Peer-Reviewed Papers
15

References

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