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MAK123 Sigma-Aldrich

α-Glucosidase Activity Assay Kit

sufficient for 100 colorimetric tests

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Properties

Related Categories Assay Kits by Specialty Area, Carbohydrate Metabolism Assay Kits, Cell Biology, Endocrinology - Diabetes, Enzymatic Activity Assay Kits,
usage   sufficient for 100 colorimetric tests
storage temp.   −20°C
Gene Information   human ... GAA(2548)
mouse ... GAA(14387)
rat ... GAA(367562)

Description

General description

α-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing a(1→4)-linked D-glucose residues with the release of D-glucose. Defects in α-glucosidase have been implicated in Pompe disease and diabetes.

Application

α-Glucosidase Activity Assay Kit has been used to measure the activity of a-glucosidase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of α-glucosidase activity in biological samples.

Principle

In this assay,α-glucosidase activity is determined by a reaction in which α-glucosidase hydrolyzes p-nitrophenyl-α-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the α-glucosidase activity present

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

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Peer-Reviewed Papers
15

References

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