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MAK134 Sigma-Aldrich

Ketone Body Assay

sufficient for 200 tests (UV)

  •  NACRES NA.84

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Properties

Related Categories Assay Kits For Studying Fatty Acid Metabolism, Assay Kits by Specialty Area, Blood and Urine Chemistry Assay Kits, Cancer Metabolism, Cancer Research,
usage   sufficient for 200 tests (UV)
feature   This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.
storage temp.   −20°C

Description

General description

Ketone bodies, 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc), are produced in the liver primarily from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol or salicylate intoxication, and inborn errors of metabolism.

Application

Ketone Body Assay has been used to measure the release of ketone bodies in the human liver cancer cell line HepG2 culture medium.

Features and Benefits

Compatible with high-throughput handling systems

Suitability

Suitable for the detection of ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) in serum, plasma, urine, and other biological samples.

Principle

In this kit, AcAc and BOH levels are determined using an enzymatic assay based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance measured at 340 nm is directly related to either the AcAc and BOH concentrations.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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