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MAK151 Sigma-Aldrich

Fluorometric Thiol Assay Kit

sufficient for 200 fluorometric tests

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Description

General description

Thiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The disulfide bonds generated when the thiol groups of two cysteine residues are oxidized contribute to the tertiary or quatenary structure of a protein.

Application

Fluorometric Thiol Assay Kit has been used to determine the lipid peroxidation expressed as thiols in samples.

Suitability

This kit is suitable for the detection of thiols in a variety of different biological samples including plasma, urine, and cell extracts. This kit is not optimized for measuring thiol groups in proteins.

Principle

The Fluorometric Thiol Quantitation Kit provides an ultrasensitive assay to quantitate the thiol content of small molecules. The proprietary thiol sensor generates a strongly fluorescent adduct (λex = 490/λem = 520 nm) upon reacting with a thiol-containing compound. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 μL assay volume (10 nM). The assay can be performed conveniently in either a 96 or 384 multiwell plate format.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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