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MAK189 Sigma-Aldrich

A-KG Dehydrogenase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym: α-KG Dehydrogenase Activity Assay Kit, α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, AKGDH Assay Kit, Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, OGDC Activity Assay Kit, Oxoglutarate Dehydrogenase Assay Kit

  •  NACRES NA.84

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Description

General description

α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is a highly regulated Krebs cycle enzyme, forms the E1 subunit of the mitochondrial 2-oxoglutarate dehydrogenase complex. It catalyzes the conversion of α-ketoglutarate to succinyl-CoA and produces NADH directly providing electrons for the respiratory chain. Decreased α-KGDH complex activity has been associated with oxidative stress and neurodegenerative disorders such as Alzheimer′s and Parkinson′s diseases.

Application

α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of α-ketoglutarate dehydrogenase in samples.

Suitability

Suitable for the detection of α-ketoglutarate dehydrogenase activity in samples such as tissue, cells, and purified mitochondria.

Principle

The α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a simple procedure for measuring α-ketoglutarate dehydrogenase activity in a variety of tissues, cells, and isolated mitochondria. α-KGDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of α-ketoglutarate dehydrogenase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.5 at 37 °C.

Kit component only

Description

Product #

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KGDH Assay Buffer    
KGDH Substrate    
KGDH Developer    
NADH Standard    
KGDH Positive Control    
Safety & Documentation

Safety Information

Symbol 
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Danger
Hazard statements 
Precautionary statements 
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NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

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