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MAK195 Sigma-Aldrich

Lipolysis (Adipocyte) Kit

Sufficient for 5 g of tissue

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Description

General description

Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.

Suitability

This kit is suitable for the isolation of adipocytes from tissue and the measurement of lipolysis.

Principle

The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λex = 535/ λem = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present.

Kit component only

Description

Product #

Add to Cart

Collagenase (0.2%)    
Collagenase Stop Buffer    
Adipocyte Wash Buffer    
Adipocyte Lipolysis Buffer    
Glycerol Assay Buffer    
Glycerol Probe, in DMSO    
Glycerol Enzyme Mix    
Glycerol Standard, 100 mM    
Isoproterenol, 10 mM    
Cell Strainer    
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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