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MAK215 Sigma-Aldrich

High Sensitivity Lipolysis Assay Kit

Sufficient for 100 Fluorometric tests

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Description

General description

Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.

Suitability

Highly sensitive kit suitable for the measurement of lipolysis in 3T3-L1 and adipocytes.

Principle

The High Sensitivity Lipolysis (3T3-L1) Fluorometric Assay Kit contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent product (λex = 535/ λem = 587 nm) proportional to the amount of glycerol present.

Kit component only

Description

Product #

Add to Cart

Lipolysis Assay Buffer    
Lipolysis Wash Buffer    
Glycerol Assay Buffer    
High-Sensitivity Probe, in DMSO    
Glycerol Enzyme Mix    
Glycerol Developer    
Glycerol Standard, 100 mM    
Isoproterenol, 10 mM    
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles
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