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MAK334 Sigma-Aldrich

Antioxidant Assay Kit

sufficient for 100 colorimetric or fluorometric tests



General description

An Antioxidant is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer′s disease, Parkinson′s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline. Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery.

The Antioxidant Assay Kit’s improved assay measures total antioxidant capacity in which Cu2+ is reduced by antioxidant to Cu+. The resulting Cu+ specifically forms a colored complex with a dye reagent. The color intensity at 570nm is proportional to TAC in the sample. The kit uses 20 μL of sample and has a linear detection range from 1.5 to 1000 μM Trolox equivalents.

Features and Benefits

Compatible with high-throughput handling systems.


This kit is suitable for antioxidant determination in serum, plasma, urine, saliva and other biological samples, food and beverages. The kit can also be utilized in drug discovery/pharmacology for studying the effects of drugs on TAC.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


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