• USA Home
  • MDQ1 - Imprint® Methylated DNA Quantification Kit

EMAIL THIS PAGE TO A FRIEND
MDQ1 Sigma-Aldrich

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

  •  NACRES NA.54

Purchase

Description

General description

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Application

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Features and Benefits

• ELISA based format
• Procedure completes in 4 easy to follow steps
• All reagents supplied including control methylated DNA
• Detection limit is 5 ng of fully methylated DNA
• Input DNA may be as low as 10-200 ng
• Procedure completes in 3.5 hours
• 96 well format allows option of studying single samples or high-throughput studies
• Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
• May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit component also available separately

Description

Product #

Add to Cart

Methylated Control DNA, 50 ng/μL SDS M8570

Kit component only

Description

Product #

Add to Cart

10x Wash Buffer    
DNA Binding Solution    
Block Solution    
5mC Capture Antibody    
Detection Antibody    
Developing Solution    
Stop Solution    
8 well Assay Strips    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can I use chloroform to extract my DNA before using Product MDQ1, Imprint® Methylated DNA Quantification Kit?
Choroform extraction should not interfere with the protocol as long as the DNA was not eluted with the chloroform.
When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, do I need to use all the wells in the plate at once?
No, you can use as many wells as needed.You will want to ensure that all the wells that are not being used are covered well with Parafilm, Saran Wrap or some other plate sealer.
What is the detection limit of Product MDQ1, Imprint® Methylated DNA Quantification Kit?
The detection limit of this kit is 5 nanograms of fully methylated DNA.
When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, can I dilute the entire antibody at once and save it for later?
No. A fresh dilution of the antibody should be prepared each time the kit is used. Diluting the antibody all at once will decrease its stability and it's effectiveness in the kit.
Are there any species restrictions for Product MDQ1, Imprint® Methylated DNA Quantification Kit?
The antibody works on 5-me-C, so any species with DNA that is methylated at the 5 position of cytidine residues will work.
Is Product MDQ1, Imprint® Methylated DNA Quantification Kit, shipped in two parts?
Yes,Product MDQ1, Imprint® Methylated DNA QuantificationKit, is shipped in 2 parts. Part1 contains, 2-8 °C components (W4267, D8568, C3493, D8818, D8693, S3447, and A4231) and Part 2 contains -20 °C components (M8570 and B6561).
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

Global DNA Methylation Quantification

The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 6
Keywords: Capture ELISA, Digestions, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Methylations

Protocols

Imprint Methylated DNA Quantification

Note: Storage Temperature - see Storage/Stability section for individual component storage conditions
Keywords: Cardiovascular, Degradations, Diseases, Gene expression, Methylations, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

H9161 Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
H9286 Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit, 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution
H7162 Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
H0913 Anti-acetyl-Histone H3 (Ac-Lys9) antibody, Mouse monoclonal, clone AH3-120, purified from hybridoma cell culture
H2663 Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal, clone HDAC2-62, purified from hybridoma cell culture
R4279 Anti-RbAp46, C-terminal antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
MOD50 Imprint® DNA Modification Kit, For bisulfite DNA conversion & purification
H7789 Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
CHP1 Imprint® Chromatin Immunoprecipitation Kit, Complete ChIP reaction in 6 hours in flexible strip well format
SH3011 MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Epigenetic Regulators
M8570 Methylated Control DNA, 50 ng/μL

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?