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MIDCLARET Sigma-Aldrich

CellVue® Claret Far Red Fluorescent Cell Linker Midi Kit for General Membrane Labeling

Distributed for Phanos Technologies

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Description

Application

CellVue Claret kit fluoresces in the far red and may be used for general cell membrane labeling including in vitro cell labeling, in vitro lymphocyte proliferation studies,† in vivo cytotoxicity assays,† and also as a second color for monitoring cell-cell membrane transfer.†

Biochem/physiol Actions

The CellVue Claret Fluorescent Cell Linker kit uses a proprietary membrane labeling technology to stably incorporate a far red fluorescent dye with long aliphatic tails (CellVue Claret) into lipid regions of the cell membrane.†

Linkage

For additional technical details on Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit PKH and CellVue®; Claret Fluorescent Cell Linker Dyes.

Packaging

This kit provides enough dye for up to 50 samples of 20 million cells each and enough diluent for up to 30 samples of 20 million cells each when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Legal Information

Distributed for Phanos Technologies, Inc.
Patent Information

CellVue is a registered trademark of PTI Research, Inc.

Kit component only

Description

Product #

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CellVue® Claret Dye, for far red fluorescent cell labeling, 1 mM in ethanol, Distributed for Phanos Technologies 2 x 0.1 mL    
Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Cell Tracking Lipophilic Membrane Dyes

Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune c...
Katharine A. Muirhead§, Joseph D. Tario, Jr.†, Paul K. Wallace†
Biowire Spring 2011 — Cell Lines — Models of Disease
Keywords: Adhesion, Asymmetric synthesis, Cancer, Capillary electrophoresis, Cell disruption, Cell division, Cell proliferation, Clinical, Flow cytometry, Gene expression, Immunology, Polymerase chain reaction, Solvents, Titrations

Choosing Lipophilic Dye Kit for Cell Tracking

From our library of Articles, Sigma-Aldrich presents Choosing Lipophilic Dye Kit for Cell Tracking
Keywords: Antivirals, Buffers, Cancer, Capillary electrophoresis, Cell disruption, Cell proliferation, Clinical, Flow cytometry, Immunology, Molecular probes

PKH Bibliography

U.S. Patent 5,665,328 Distributed for Phanos Technologies, Inc. CellVue is a registered trademark of PTI Research, Inc. used under license.
Keywords: AGE, Adhesion, Amplification, Angiogenesis, Antibiotics, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Biomaterials, Cancer, Capillary electrophoresis, Cell culture, Cell disruption, Cell division, Cell proliferation, Central Nervous System, Clinical, Eliminations, Endocrinology, Filtration, Flow cytometry, Gene expression, Growth factors, Immobilization, Immunology, Inflammation, Ion-exchange chromatography, Ligands, Magnetic resonance imaging, Mass spectrometry, Metabolites, Microscopy, Molecular biology, Reductions, Rheumatology, Transduction, Western blot

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects. Fluorescent linker kits are effective for a v...
Keywords: Cell division, Immunofluorescence

Protocols

Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes

Exosomes are microscale extracellular vesicles that are present in most somatic fluids including blood and urine, as well as the media of cell cultures. These-lipid-bearing microvesicles range in siz...
Keywords: Cancer, Flow cytometry

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
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Peer-Reviewed Papers
15

References

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