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MIDI26 Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies



General description

This kit provides enough dye for up to 50 samples of 20 million cells each and enough diluent for up to 30 samples of 20 million cells each when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.


This kit is for general cell membrane labeling, including for in vitro cell labeling, in vitro proliferation studies, and long term in vivo cell tracking.


For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of PTI Research, Inc.

Standard component


Product #

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Diluent C 60 mL    
PKH26 Cell Linker in Ethanol .2 mL    
Safety & Documentation

Safety Information

GHS02  GHS02
Signal word 
Hazard statements 
Precautionary statements 
UN 3316 9
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

PKH Bibliography

U.S. Patent 5,665,328 Distributed for Phanos Technologies, Inc. CellVue is a registered trademark of PTI Research, Inc. used under license.
Keywords: AGE, Adhesion, Amplification, Angiogenesis, Antibiotics, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Biomaterials, Cancer, Capillary electrophoresis, Cell culture, Cell disruption, Cell division, Cell proliferation, Central Nervous System, Clinical, Eliminations, Endocrinology, Filtration, Flow cytometry, Gene expression, Growth factors, Immobilization, Immunology, Inflammation, Ion-exchange chromatography, Ligands, Magnetic resonance imaging, Mass spectrometry, Metabolites, Microscopy, Molecular biology, Reductions, Rheumatology, Transduction, Western blot

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects. Fluorescent linker kits are effective for a v...
Keywords: Cell division, Immunofluorescence


Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes

Exosomes are microscale extracellular vesicles that are present in most somatic fluids including blood and urine, as well as the media of cell cultures. These-lipid-bearing microvesicles range in siz...
Keywords: Cancer, Flow cytometry

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