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PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

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For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Application

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Legal Information

CellVue is a registered trademark of PTI Research, Inc.

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PKH67 Dye 2 x 0.1 mL    

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Diluent C 6 x 10 mL    
Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Cell Tracking Lipophilic Membrane Dyes

Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune c...
Katharine A. Muirhead§, Joseph D. Tario, Jr.†, Paul K. Wallace†
Biowire Spring 2011 — Cell Lines — Models of Disease
Keywords: Adhesion, Asymmetric synthesis, Cancer, Capillary electrophoresis, Cell disruption, Cell division, Cell proliferation, Clinical, Flow cytometry, Gene expression, Immunology, Polymerase chain reaction, Solvents, Titrations

Choosing Lipophilic Dye Kit for Cell Tracking

From our library of Articles, Sigma-Aldrich presents Choosing Lipophilic Dye Kit for Cell Tracking
Keywords: Antivirals, Buffers, Cancer, Capillary electrophoresis, Cell disruption, Cell proliferation, Clinical, Flow cytometry, Immunology, Molecular probes

PKH Bibliography

U.S. Patent 5,665,328 Distributed for Phanos Technologies, Inc. CellVue is a registered trademark of PTI Research, Inc. used under license.
Keywords: AGE, Adhesion, Amplification, Angiogenesis, Antibiotics, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Biomaterials, Cancer, Capillary electrophoresis, Cell culture, Cell disruption, Cell division, Cell proliferation, Central Nervous System, Clinical, Eliminations, Endocrinology, Filtration, Flow cytometry, Gene expression, Growth factors, Immobilization, Immunology, Inflammation, Ion-exchange chromatography, Ligands, Magnetic resonance imaging, Mass spectrometry, Metabolites, Microscopy, Molecular biology, Reductions, Rheumatology, Transduction, Western blot

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects. Fluorescent linker kits are effective for a v...
Keywords: Cell division, Immunofluorescence

Protocols

Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes

Exosomes are microscale extracellular vesicles that are present in most somatic fluids including blood and urine, as well as the media of cell cultures. These-lipid-bearing microvesicles range in siz...
Keywords: Cancer, Flow cytometry

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Cell Division Monitoring using Cell Tracking Dyes [VIDEO]

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.1-5 Althou...
Keywords: Cancer, Cell proliferation, Clinical, Flow cytometry

Peer-Reviewed Papers
15

References

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PKH67GL PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Distributed for Phanos Technologies

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