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MIRAP00015 Sigma-Aldrich

MystiCq® microRNA qPCR Assay Primer




Related Categories Molecular Biology, PCR/Amplification, Quantitative PCR, miRNA qPCR Assay Primers hsa-let-00 to hsa-miR-517, microRNA Quantitative PCR More...
form   liquid
concentration   10 mg/mL
mp   ~75.5 °C
Sanger mature/minor accession no.   MIMAT0000416
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MIR1-2(406905)


General description

The MystiCq microRNA qPCR Assay Primer is an integral part of the MystiCq microRNA qPCR Assay System. It has been designed to target specific microRNAs with the MystiCq Universal PCR primer and the MystiCq microRNA SYBR Green qPCR ReadyMix on cDNA templates generated by the MystiCq microRNA cDNA Synthesis Mix kit.

Features and Benefits

Features and Benefits
• Proprietary design features of assay primers allow for specificity towards mature microRNAs vs. precursor-microRNAs
• Extensive design and testing of oligo-dT adapter primer to incorporate complementary Universal PCR Primer sequence
• No self-complementarity or primer dimer artifacts with the Universal PCR Primer
• Optimized primer Tm designed to match the Universal PCR Primer
• Universal cycling conditions ensures robust amplification for all assays in profiling experiments
• Optimized PCR product Tm (75-78° C)

Other Notes

Compatible with mmu-miR-1.

Legal Information

MystiCq is a registered trademark of Qiagen Beverly Inc.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles

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We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers


Related Products

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Add to Cart

MIRRM03 MystiCq® microRNA® SYBR® Green qPCR ReadyMix, Formulation for miRNA RT-qPCR on iQ Bio-Rad platforms
MIRRM00 MystiCq® microRNA® SYBR® Green qPCR ReadyMix, Formulated for systems that do not require normalization
MIRRM02 MystiCq® microRNA® SYBR® Green qPCR ReadyMix, with ROX, formulation for miRNA RT-qPCR
MIRRM01 MystiCq® microRNA® SYBR® Green qPCR ReadyMix, Low ROX formulation for miRNA RT-qPCR
MIRUP MystiCq® Universal PCR Primer
MIRRT MystiCq® microRNA cDNA Synthesis Mix

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