• USA Home
  • MIRRM00 - MystiCq® microRNA® SYBR® Green qPCR ReadyMix

EMAIL THIS PAGE TO A FRIEND
MIRRM00 Sigma-Aldrich

MystiCq® microRNA® SYBR® Green qPCR ReadyMix

Formulated for systems that do not require normalization

  •  NACRES NA.55

Purchase

Description

General description

The MystiCq microRNA SYBR Green qPCR ReadyMix is one of the integral components of the MystiCq microRNA qPCR Assay System. This ReadyMix is a specially-formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.

Application

MystiCq microRNA SYBR Green qPCR ReadyMix is compatible with the following platforms: Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MiniOpticon, Bio-Rad/MJ Chromo4, Bio-Rad/MJ Opticon 2, Bio-Rad/MJ Opticon, Cepheid SmartCycler®, Eppendorf Mastercycler® ep realplex, Eppendorf Mastercycler ep realplex2 s, Illumina Eco qPCR, Qiagen/Corbett Rotor-Gene® 3000, Qiagen/Corbett Rotor-Gene 6000, Qiagen/Corbett Rotor-Gene Q, Roche LightCycler® 480.

Features and Benefits

• Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
• 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
• Passive reference dye (if required) included at the optimal concentration

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL

Legal Information

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

LightCycler is a registered trademark of Roche

Mastercycler is a registered trademark of Eppendorf AG

MystiCq is a registered trademark of Qiagen Beverly Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen GmbH

SYBR is a registered trademark of Life Technologies

SmartCycler is a registered trademark of Cepheid, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?