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MIRRM02 Sigma-Aldrich

MystiCq® microRNA® SYBR® Green qPCR ReadyMix

with ROX, formulation for miRNA RT-qPCR

  •  NACRES NA.55



General description

The MystiCq microRNA SYBR Green qPCR ReadyMix is one of the integral components of the MystiCq microRNA qPCR Assay System. This ReadyMix is a specially-formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.


MystiCq microRNA SYBR Green qPCR ReadyMix, ROX, is compatible with the following platforms: Applied Biosystems 5700, Applied Biosystems 7000, Applied Biosystems 7300, Applied Biosystems 7700, Applied Biosystems 7900, Applied Biosystems 7900 HT Fast, Applied Biosystems 7900 HT, Applied Biosystems StepOnePlus, Applied Biosystems StepOne.

Features and Benefits

• Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
• 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
• Passive reference dye (if required) included at the optimal concentration


Default reaction volume is 50 μL

100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL

Legal Information

MystiCq is a registered trademark of Qiagen Beverly Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

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Peer-Reviewed Papers


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