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MP0030 Sigma-Aldrich

LookOut® Mycoplasma Elimination Kit

  •  NACRES NA.75

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Properties

Related Categories Assays & Reagents for Measuring Cytotoxicity, Proliferation & Viability Kits, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Cell Analysis, Cell Biology, Cell Culture,
Quality Level   200
usage   1 kit sufficient for 5 mL mycoplasma elimination
packaging   pkg of 1 kit
storage condition   protect from light
application(s)   PCR: suitable
  detection: suitable
  microbiology: suitable
storage temp.   2-8°C

Description

General description

The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.

Application

The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.

Features and Benefits

• Minimal cytotoxic properties of the reagents
• Highly effective
• Retention of normal cell characteristics

Suitability

The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.

Legal Information

LookOut is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Mycoplasma Detection and Elimination In Cell Culture

The maintenance of contamination-free cell cultures is fundamental to cell-based research. Among the most pervasive concerns is contamination with mycoplasma, a genus of bacteria whose members are ty...
Keywords: Antibiotics, Apoptosis, Cell culture, Culture media, Detection methods, Eliminations, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Metabolism, Microbiology, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Thin layer chromatography

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

PCR-based Detection of Mycoplasma Contamination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest contaminant concerns are mycoplasma contamination. Although mycoplasma do not usually kill cont...
Keywords: Amplification, Detection methods, Eliminations, Enzyme-linked immunosorbent assay, Metabolism, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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