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MP0040A Sigma-Aldrich

LookOut® Mycoplasma qPCR Detection Kit

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Properties

Related Categories Cell Analysis, Cell Biology More...
Quality Level   200
quality   Not suitable for clinical diagnostic use.
storage temp.   2-8°C

Description

General description

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit already contains Taq polymerase in the Mycoplasma Mix.

Application

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Legal Information

LookOut is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Mycoplasma Detection and Elimination

The maintenance of contamination-free cell cultures is fundamental to cell-based research. Among the most pervasive concerns is contamination with mycoplasma, a genus of bacteria whose members are ty...
Keywords: Antibiotics, Apoptosis, Cell culture, Culture media, Detection methods, Eliminations, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Metabolism, Microbiology, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Thin layer chromatography

Related Content

PCR-based Detection of Mycoplasma Contamination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest contaminant concerns are mycoplasma contamination. Although mycoplasma do not usually kill cont...
Keywords: Amplification, Detection methods, Eliminations, Enzyme-linked immunosorbent assay, Metabolism, Polymerase chain reaction, Polymerase chain reaction - quantitative

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