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MREH01 Sigma

MISSION® Target ID Library

Easy Identification of miRNA Gene Targets

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Properties

shipped in   wet ice
storage temp.   −20°C

Description

Components

Library: 100μl (approx 1μg/μl), frozen liquid, store at -10°C to -25°C.
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

General description

The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense.

The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.

The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing

Download deep sequencing dataof target ID library.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

The Screening Advantage
Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

RNAi Tools for Epigenetics Research

RNA interference (RNAi) is a relatively new technology that is revolutionizing the way researchers study gene expression. RNAi is a highly conserved gene silencing mechanism that occurs in response t...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 17–18
Keywords: Cancer, Cell culture, Cell division, Epigenetics, Gene expression, PAGE

The MISSION® Target ID Library for Human miRNA Target Identification and Discovery

One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are...
Keywords: AGE, Amplification, Apoptosis, Cancer, Cell culture, Cloning, Degradations, Detection methods, Digestions, Electrophoresis, Environmental, Fractionation, Gel electrophoresis, Gene expression, Genomics, Immunoprecipitation, Melting, Methods, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA immunoprecipitation, Reductions, Sequences, Sequencing, Support, Transfection, Western blot, transformation

Related Content

Genome-wide Screen for miRNA Targets Using the MISSION® Target ID Library [VIDEO]

The MISSION Target ID Library is designed to assist in discovery and identification of microRNA (miRNA) targets. The MISSION Target ID Library is a plasmid-based, genome-wide cDNA library cloned into...
Keywords: PAGE, Sequencing

Peer-Reviewed Papers
15

References

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