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MSQC10 Sigma-Aldrich

SILuMAb Mouse Stable-Isotope Labeled Monoclonal Antibody

from mouse, recombinant, expressed in CHO cells

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Properties

Related Categories Antibody Characterization and Analysis, Biochemicals and Reagents, Mass Spectrometry, Molecular Biology, Peptide and Protein Standards for Mass Spectrometry Analysis,
biological source   mouse
recombinant   expressed in CHO cells
antibody product type   primary antibodies
assay   ≥90% (SDS-PAGE)
packaging   vial of 100 μg
shipped in   wet ice
storage temp.   −20°C

Description

General description

SILuMAb Mouse is a recombinant, stable isotope-labeled, mouse monoclonal antibody which incorporates [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in CHO cells, SILuMAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies.

SILuMAb Mouse is an IgG1 antibody with a kappa light chain. Because of overlap with common sequences in the Fc region with candidate murine antibodies, SILuMAb Mouse provides universal utility, and thus, eliminates the need to produce candidate-specific internal standards.

Physical form

Each vial of SILuMAb Mouse contains the labeled antibody lyophilized from a solution of phosphate buffered saline. Vial content was determined by measuring A280 and using an extinction coefficient (E0.1%) of 1.4.

Preparation Note

Produced utilizing enriched media containing stable isotope labeled amino acids such as [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in CHO cells, SILuMAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies

Reconstitution

SILuMAb Mouse recovery is maximized when 0.1% formic acid is used for reconstitution of the lyophilized product.
Reconstitution with other solvents may reduce recovery. Do not freeze after reconstitution.

• Briefly centrifuge the vial at ~10,000 × g to collect the product at the bottom of the vial.
• Add 500 μL of ultrapure water containing 0.1% formic acid to the vial.
• Mix the contents by gently inverting the vial a minimum of 5 times.
• Allow the vial to stand at room temperature for at least 15 minutes and repeat mixing by inversion.

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
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