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MSQC8 Sigma-Aldrich

SigmaMAb Antibody Drug Conjugate (ADC) Mimic

Antibody Cysteine-Fluorophore Conjugate Standard

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Properties

Related Categories Antibody Characterization and Analysis, Biochemicals and Reagents, Mass Spectrometry, Molecular Biology, Peptide and Protein Standards for Mass Spectrometry Analysis,
recombinant   expressed in CHO cells
antibody product type   primary antibodies
form   lyophilized powder
shipped in   wet ice
storage temp.   −20°C

Description

Application

Poster: Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk

Poster: LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic

Poster: LC-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC Mimic

General description

SigmaMab Antibody Drug Conjugate (ADC) Mimic, is a non-toxic drug mimic that is utilized as a standard for mass spectrometry and high performance liquid chromatography. The ADC Mimic conjugates SigmaMab (MSQC4), an IgG1 monoclonal antibody, to dansyl fluorophores via an LC-SMCC crosslinker. This dansyl-LC-SMCC attachment serves as the substitution for the toxic payload of a real ADC.

The ADC Mimic allows for safer and more consistent testing, making it the optimal product over real ADCs. It is also a more affordable product compared to real ADCs. With the help of this non-toxic product, LC-MS processes can be developed in a safer environment.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

ADC Mimic Enables LC-MS Method Development

A non-toxic Cys-conjugated ADC-mimic characterized by top-down, middle-out, and bottom-up LC-MS methods.
Kevin Ray, Ben Cutak, Brian Gau
MilliporeSigma, St. Louis, MO
Keywords: Alkylations, Bacterial conjugations, Chromatography, Genetics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Reductions, Size-exclusion chromatography

ADC-Mimic for LC-UV-MS Method Development

Antibody-drug conjugates (ADC) represent a rapidly emerging class of biotherapeutic molecules, which harness the specificity of monoclonal antibodies (mAb) to selectively deliver highly potent cytoto...
Benjamin Cutak, Brian Gau, Nicolas Caffarelli, and Kevin Ray
MilliporeSigma, St. Louis, MO
Keywords: Bacterial conjugations, Chromatography, Fractionation, High performance liquid chromatography, Mass spectrometry, Reductions, Separation

LC/MS Analysis of Reduced SigmaMAb ADC Mimic on BIOshell™ IgG 1000 Ã… C4

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Reduced SigmaMAb ADC Mimic on BIOshell™ IgG 1000 Ã… C4
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass selective detector, Mass spectrometry, Pharmaceutical, Substitutions

Non-Toxic ADC-Mimic for LC-MS Method Development

ADCs conjugated at Cys residues of inter-chain disulfide bonds give mAbs bearing 0-4 pairs of drug-linkers. Characterizing this distribution is paramount to understanding ADC efficacy and process con...
Brian Gau, Ben Cutak, Pegah Jalili, Kevin Ray
MilliporeSigma, St. Louis, MO
Keywords: Bacterial conjugations, Buffers, Filtration, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Purification, Size-exclusion chromatography

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What happens when you combine state-of-the-art technology with scientific prowess? Full-length protein standards with the highest purity and isotopic incorporation in the industry. Stable isotope-lab...
Keywords: Atomic absorption spectroscopy, Buffers, Digestions, Mass spectrometry, PAGE, Phosphorylations

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