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MSSAFE Sigma-Aldrich

MS-SAFE Protease and Phosphatase Inhibitor

Synonym: Mass Spectrometry Safe Protease and Phosphatase Inhibitor Cocktail

  •  NACRES NA.77

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Description

General description

Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:
• Serine, cysteine and aspartic proteases, and metalloproteases
• Tyrosine, serine/threonine, acid and alkaline phosphatases
All inhibitors and fillers were chosen so as not to interfere with LC-MS analyses. Any inhibitors capable of covalent, irreversible protein modification were avoided. This cocktail is EDTA-free. All inhibitors were selected to allow facile downstream sample processing, such as immobilized metal affinity chromatography (IMAC) for His-tagged protein purification and, uniquely, phosphopeptide enrichment.

Specificity

Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases

Application

MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.

Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry.

Packaging

1 vial in glass bottle

Components

Protease inhibitors:
• Bestatin hydrochloride
• Leupeptin
• Phosphoramidon disodium salt
• Pepstatin A
• Elastatinal
• Aprotinin
• Nafamostat mesylate
• Antipain
Phosphatase inhibitors
• Okadaic acid
• Sodium fluoride
• Sodium orthovanadate
• Bromotetramisole oxalate
Fillers
• β-lactose
• DL-leucine

Quantity

One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.

Physical form

Lyophilized powder that is water-soluble

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Protease Inhibitor Cocktails References

Our portfolio of protease inhibitor cocktails are frequently cited in peer-reviewed scientific literature, suggesting their superior quality and excellent track record. This page lists a sampling of ...
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Peer-Reviewed Papers
15

References

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