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MSST0021 Sigma-Aldrich

SILuProt CRP C-reactive protein human

recombinant, expressed in HEK 293 cells, 13C and 15N-labeled

Synonym: PTX1, pentaxin, pentraxin

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Properties

Related Categories Biochemicals and Reagents, Proteins and Derivatives, SILuProt Protein Standards for Quantitative Mass Spectrometry More...
Quality Level   200
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥98% (SDS-PAGE)
form   liquid
potency   ≥98% Heavy amino acids incorporation efficiency by MS
application(s)   mass spectroscopy (MS): suitable
suitability   suitable for mass spectrometry (standard)
UniProt accession no.   P02741
storage temp.   −20°C
Gene Information   human ... PTX3(5806)

Description

General description

SILuProt CRP is a recombinant, stable isotope-labeled human CRP which incorporates [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in human 293 cells, it is designed to be used as an internal standard for bioanalysis of CRP in mass-spectrometry. SILuProt CRP is a protein consisting of 226 amino acids (including a C-terminal polyhistidine and FLAG® tags), with a molecular weight of ~25 kDa.


Suggested Quantitative Analysis Parameters
(MRM settings provided for three suggested peptides)

Biochem/physiol Actions

CRP is a hepatically derived pentraxin. It exists mainly as a pentamer in a cyclic structure with 5 identical 23-kD subunits in the blood. In addition to its role as an "acute phase" protein or first-line defense molecule against pathogenic organisms, baseline levels of CRP correlate positively to adverse cardiovascular events. CRP is therefore being considered a “risk factor” for cardiovascular disease. In humans, treatment with statin therapy reduces levels of both LDL-C and CRP, and concurrently there is a reduction in the number of cardiovascular events. A recent study suggests that patients with elevated basal levels of CRP are at an increased risk of diabetes, in addition to cardiovascular disease. A study of over 700 nurses showed that those in the highest quartile of trans fat consumption had blood levels of CRP that were 73% higher than those in the lowest quartile.

Physical form

Supplied as a solution of phosphate buffered saline and 25% glycerol.

Legal Information

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This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com.

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

SILu is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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