• USA Home
  • MTOX1010 - HepaRG 5F Control Cells

EMAIL THIS PAGE TO A FRIEND
MTOX1010 Sigma-Aldrich

HepaRG 5F Control Cells

1 vial

  •  NACRES NA.24

Purchase

Properties

Related Categories ADME/Tox Products, Cell Biology, Cell Line Models, Liver Drug Metabolism Knockout Models, Liver Drug Transporter/Toxicity Models More...
Quality Level   200
biological source   human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
storage temp.   −196°C

Description

General description

This product consists of ZFN engineered HepaRG 5F clone control cells. They are intended for use as both control cells for HepaRG knockout cells as well as for a wide variety of liver cell based assays. Hepa RG cells display hepatocyte-like functions and can be a replacement to primary human hepatocytes in a number of drug metabolism, disposition and genotoxicity studies.

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

HepaRG 5F Control Cells has been used in a gene expression assay to study the effect of pregnane X receptor on the induction of human cytochrome P450 3A4 by the irreversible myeloperoxidase inactivator PF-06282999.

See technical bulletins for detailed protocols

Features and Benefits

Sigma′s HepaRG 5F Clone exhibits improved growth characteristics and enhanced functionality, expressing a large panel of drug metabolism enzymes; including cytochrome P450 enzymes CYP1A2, CYP2B6, CYP2C8/9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4, nuclear receptors AhR, PXR, CAR, LXR, and FXR, and phase II enzymes UGT, SULT, NAT and GST.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Legal Information

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

High-throughput Imaging of a Unique Continuous Flow Microfluidics Plate

The continued drive towards cell based assays that better mimic the in vivo environment has led to innovative cell culture systems such as the SciFlow™ 1000 Fluidic Culture System, which utilizes cap...
Keywords: Atomic absorption spectroscopy, Cell attachment, Cell culture, Culture media, Environmental, Indicators, Metabolites

Predict Cytotoxic Effect While Generating More in Vivo-Like Data

The next step in cell-based assays is to reproduce organ and living system complexity for more accurate assessment of adaptive vs. toxic mechanisms of compound treatment. A dynamic exposure scenario ...
Keywords: Atomic absorption spectroscopy, Cell culture, Culture media, Metabolites

Serial Multiwell Gradient Exposures for Analyzing Effects of Changing Parent-Metabolite Ratios

The SciFlow System dynamic fluid gradients enable direct observation of the cellular effects of changing parent-metabolite ratios in cell culture.
Keywords: Apoptosis, Atomic absorption spectroscopy, Cell culture, Culture media, Detergents, Indicators, Metabolites

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic ci...
David C. Thompson, Ph.D, R&D Manager, Sigma® Life Science and Michael D. Mitchell, Product Manager, Sigma® Life Science
Biofiles Vol. 8, No. 12
Keywords: Absorption, Cancer, Clinical, Eliminations, Genetic, Metabolic Pathways, Metabolism, Pharmaceutical, Polymorphisms

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?