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MTOX1012 Sigma-Aldrich

CAR Knockout HepaRG Cells

1 vial

  •  NACRES NA.81



Related Categories ADME/Tox Products, Cell Biology, Cell Line Models, Liver Drug Metabolism Knockout Models
Quality Level   200
biological source   human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
storage temp.   −196°C
Gene Information   human ... ABCB1(1346307)


General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG CAR KO cells express the major xenobiotic sensors (PXR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.


See technical bulletin for detailed protocols

Features and Benefits

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

• Sigma′s HepaRG CAR Knockout (KO) allows conclusive identification of CYP2B6 induction through CAR activation.

Zinc finger nucleases (ZFN) mediated Knock-out of NR1I3 (CAR) gene.

• The frame-shift mutation of NR1I3 gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.


Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Legal Information

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

Safety & Documentation

Safety Information

UN 3245 9
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


High-throughput Imaging of a Unique Continuous Flow Microfluidics Plate

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Predict Cytotoxic Effect While Generating More in Vivo-Like Data

The next step in cell-based assays is to reproduce organ and living system complexity for more accurate assessment of adaptive vs. toxic mechanisms of compound treatment. A dynamic exposure scenario ...
Keywords: Atomic absorption spectroscopy, Cell culture, Culture media, Metabolites

Serial Multiwell Gradient Exposures for Analyzing Effects of Changing Parent-Metabolite Ratios

The SciFlow System dynamic fluid gradients enable direct observation of the cellular effects of changing parent-metabolite ratios in cell culture.
Keywords: Apoptosis, Atomic absorption spectroscopy, Cell culture, Culture media, Detergents, Indicators, Metabolites

Peer-Reviewed Papers


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