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MTOX1013 Sigma-Aldrich

HepaRG Cells AHR (-/-)

one vial, from human female liver(hepatocarcinoma and hepatitis C tumor)

  •  NACRES NA.81

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Properties

Related Categories ADME/Tox Products, Cell Biology, Cell Line Models, Liver Drug Metabolism Knockout Models
Quality Level   200
biological source   human female liver (hepatocarcinoma and hepatitis C tumor)
OMIM accession no.  
Gene Information   human ... AHR(196)

Description

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG AHR knockout cells express the major xenobiotic sensors (PXR and CAR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

See technical bulletin for detailed protocols

Features and Benefits

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG AHR Knockout (KO) allows conclusive identification of CYP1A1 and CYP1B1 induction through AHR activation.

Zinc finger nucleases (ZFN) mediated Knockout of AHR (AHR) gene.
• The frame-shift mutation of AHR gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Legal Information

Exhibit 1: ADME/Tox cell lines license
Exhibit 2: HepaRG limited use license

HepaRG is a trademark of BioPredic International company

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

High-throughput Imaging of a Unique Continuous Flow Microfluidics Plate

The continued drive towards cell based assays that better mimic the in vivo environment has led to innovative cell culture systems such as the SciFlow™ 1000 Fluidic Culture System, which utilizes cap...
Keywords: Atomic absorption spectroscopy, Cell attachment, Cell culture, Culture media, Environmental, Indicators, Metabolites

Predict Cytotoxic Effect While Generating More in Vivo-Like Data

The next step in cell-based assays is to reproduce organ and living system complexity for more accurate assessment of adaptive vs. toxic mechanisms of compound treatment. A dynamic exposure scenario ...
Keywords: Atomic absorption spectroscopy, Cell culture, Culture media, Metabolites

Serial Multiwell Gradient Exposures for Analyzing Effects of Changing Parent-Metabolite Ratios

The SciFlow System dynamic fluid gradients enable direct observation of the cellular effects of changing parent-metabolite ratios in cell culture.
Keywords: Apoptosis, Atomic absorption spectroscopy, Cell culture, Culture media, Detergents, Indicators, Metabolites

Peer-Reviewed Papers
15

References

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