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  • N0142 - Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse

N0142 Sigma

Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse

clone N52, ascites fluid



Related Categories ALS, Prion Disease, and Others, ALS, Prion Disease, and others, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   wide range
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): 1:400 using rat cerebellum
  microarray: suitable
  western blot: 1:1,000 using rat brain extract
clone   N52, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)
biological source   mouse
conjugate   unconjugated



C-terminal segment of enzymatically dephosphorylated pig neurofilament 200.


Displays a broad species cross-reactivity, recognizing both the phosphorylated and non-phosphorylated forms of this neural specific antigen using various techniques.

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RIDADR  NONH for all modes of transport
WGK Germany  3
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Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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