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N0399 Sigma-Aldrich

Anti-Nix antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-BNIP3α, Anti-BNIP3L

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Bcl-2 Family Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5-1 μg/mL using a K562 cell lysate.
species reactivity   human, mouse
mol wt   antigen 38 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   1 mg/mL
UniProt accession no.   O60238
Gene Information   human ... BNIP3L(665)
mouse ... Bnip3l(12177)

Description

General description

BCL2/adenovirus E1B 19kDa interacting protein 3-like (BNIP3L) is expressed in the outer membrane of mitochondria. The BNIP3L gene is localized on human chromosome 8p21.

Immunogen

synthetic peptide corresponding to amino acids 77-92 of an internal domain of human Nix. This peptide is identical in mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

BCL2/adenovirus E1B 19kDa interacting protein 3-like (BNIP3L) associates with proteins which are anti-apoptotic, like B-cell lymphoma 2 (Bcl-2). It also takes part in the growth of the erythroid lineage. Expression of BNIP3L is downregulated in lung cancer cells in vitro.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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