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N1127 Sigma-Aldrich

2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

Synonym: o-Nitrophenyl β-D-galactopyranoside, ONPG

  • CAS Number 369-07-3

  • Empirical Formula (Hill Notation) C12H15NO8

  • Molecular Weight 301.25

  •  Beilstein Registry Number 92207

  •  EC Number 206-716-1

  •  MDL number MFCD00063255

  •  PubChem Substance ID 24897461

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Description

General description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Packaging

1, 5, 25 g in poly bottle

500 mg in poly bottle

250 mg in glass bottle

Principle

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Reconstitution

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

Substrates

Chromogenic substrate for β-galactosidase

Application

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Same Products - New Packaging
Protocols & Articles

Articles

2-Nitrophenyl β-D-galactopyranoside

This product (ONPG) is a colorimetric substrate for β-galactosidase. This product is hydrolyzed to o-nitrophenol by the enzyme. o-Nitrophenol is yellow in alkaline solution and can be quantitated at ...
Keywords: Extinction coefficient, Melting

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Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
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Protocols

Introduction to Yeast Transformation

Yeasts are eukaryotic model systems for studies as they exhibit fast growth and have dispersed cells. They have a well-defined genetic system and a highly versatile DNA transformation system that can...
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Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Peer-Reviewed Papers
15

References

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