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N1531 Sigma-Aldrich

Denaturation Solution

for Northern and Southern blotting

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Properties

Related Categories Biochemicals, Buffer / Buffer Salts, Core Bioreagents, Life Science Reagents for Northern and Southern Blotting, Molecular Biology,
grade   for molecular biology
form   solution
storage temp.   room temp

Description

General description

Sigma’s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Application

Suitable for denaturing dsDNA before transfer to membranes during Southern procedures.

Components

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Other Notes

0.5 N NaOH, 1.5 M NaCl

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1824 8 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Peer-Reviewed Papers
15

References

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