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N1665 Sigma-Aldrich

5′-Nucleotidase human

recombinant, expressed in CHO cells, vial of 6-12 μg

Synonym: CD73, NT5E, ecto-5′-nucleotidase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Biochemicals and Reagents, Cell Biology,
Quality Level   200
recombinant   expressed in CHO cells
description   contains a C-terminal 6-His tag
assay   ≥90% (SDS-PAGE)
form   solution
enzyme activity   ≥15 units/mg protein
mol wt   ~61 kDa by SDS-PAGE (reducing)
packaging   vial of 6-12 μg
impurities   ≤1.0 EU/μg endotoxin
NCBI accession no.   NP_002517
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... NT5E(4907)

Description

General description

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

Biochem/physiol Actions

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.

5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

Unit Definition

One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.

Physical form

Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
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