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  • N218 - Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse

N218 Sigma-Aldrich

Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse

clone NOS-3F7-B11-B5, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
conjugate   unconjugated
clone   NOS-3F7-B11-B5, monoclonal
biological source   mouse
application(s)   immunohistochemistry (frozen sections): 1:100
  western blot: 1:50-1:500
species reactivity   rat, bovine, mouse
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgM
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P29476
Gene Information   mouse ... Nos1(18125)
rat ... Nos1(24598)



The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS).


bovine cerebellum NOS.

Physical form

Ascites fluid containing ≤0.1% sodium azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Neuronal nitric acid synthase (Nos1) in involved in the production of nitric oxide. Mutations in the gene encoding it increases the susceptibility to behavioral dysfunction after an exposure to alcohol in mice.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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