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  • N2643 - Anti-Nitric Oxide Synthase, Endothelial (596-609) antibody produced in rabbit

N2643 Sigma-Aldrich

Anti-Nitric Oxide Synthase, Endothelial (596-609) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-eNOS



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (frozen sections): 1:100 using acetone-fixed, frozen tissue sections of mouse heart
  microarray: suitable
  western blot: 1:10,000 using bovine lung endothelial cell extract
species reactivity   bovine, mouse, human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P29474
Gene Information   human ... NOS3(4846)
mouse ... Nos3(18127)


General description

The gene encoding endothelial nitric oxide synthase (eNOS) is localized on human chromosome 7q35-36 and has 26 exons.

eNOS encodes a 135 kDa protein and is localized in endothelial cells. Human bNOS and eNOS share 52% amino acid identity, and rat and human bNOS share 93% amino acid identity. eNOS is not restricted to the endothelium of blood vessels but exists in the epithelium of several tissues.


synthetic peptide corresponding to nitric oxide synthase (NOS) of bovine endothelial origin (eNOS, amino acids 596-609) conjugated to KLH. The immunogen sequence is identical in human eNOS.


Anti-Nitric Oxide Synthase, Endothelial (596-609) antibody produced in rabbit has been used in western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Endothelial nitric oxide synthase (eNOS) is a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated coronary artery disease.

Endothelial nitric oxide synthase (eNOS) is constitutively expressed and are dependent on Ca2+/ calmodulin for nitricoxide (NO) production.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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